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immunosorbent assay elisa kits  (R&D Systems)


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    R&D Systems immunosorbent assay elisa kits
    A Adeno-associated virus 8 (AAV8) expressing Usp25 (AAV–Usp25) was injected into mice to overexpress USP25 and subsequently induce colitis in mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the mice in four groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by <t>enzyme-linked</t> <t>immunosorbent</t> <t>assay.</t> I After using the plasmid to overexpress USP25 in NCM460 cells, the cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. J Immunofluorescence was performed to examine the expression of occludin protein in NC and USP25 overexpressing NCM460 cells with LPS treatment. * p < 0.05, ** p < 0.01 compared with control groups.
    Immunosorbent Assay Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunosorbent assay elisa kits/product/R&D Systems
    Average 95 stars, based on 224 article reviews
    immunosorbent assay elisa kits - by Bioz Stars, 2026-03
    95/100 stars

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    1) Product Images from "Ubiquitin-specific protease 25 ameliorates ulcerative colitis by regulating the degradation of phosphor-STAT3"

    Article Title: Ubiquitin-specific protease 25 ameliorates ulcerative colitis by regulating the degradation of phosphor-STAT3

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-024-07315-z

    A Adeno-associated virus 8 (AAV8) expressing Usp25 (AAV–Usp25) was injected into mice to overexpress USP25 and subsequently induce colitis in mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the mice in four groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by enzyme-linked immunosorbent assay. I After using the plasmid to overexpress USP25 in NCM460 cells, the cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. J Immunofluorescence was performed to examine the expression of occludin protein in NC and USP25 overexpressing NCM460 cells with LPS treatment. * p < 0.05, ** p < 0.01 compared with control groups.
    Figure Legend Snippet: A Adeno-associated virus 8 (AAV8) expressing Usp25 (AAV–Usp25) was injected into mice to overexpress USP25 and subsequently induce colitis in mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the mice in four groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by enzyme-linked immunosorbent assay. I After using the plasmid to overexpress USP25 in NCM460 cells, the cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. J Immunofluorescence was performed to examine the expression of occludin protein in NC and USP25 overexpressing NCM460 cells with LPS treatment. * p < 0.05, ** p < 0.01 compared with control groups.

    Techniques Used: Virus, Expressing, Injection, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Cell Culture, Immunofluorescence, Control

    A To determine the optimal LPS concentration, LPS was used to induce cellular inflammation in the human colonic epithelial cell line, NCM460. B After using lentivirus shSTAT3 to interfere with the expression of STAT3 in NCM460 cells, cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. C Immunofluorescence was performed to examine the expression of ZO-1 protein in NC and STAT3 low-expressing NCM460 cells with LPS treatment. D DSS was used to induce colitis in State fl/fl and Villin-Cre State fl/fl mice ( n = 8 per group). Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( E ), DAI score ( F ), colon length ( G ), and histopathological score ( H ). Body weight changes in mice are expressed as a percentage of the initial body weight ( E ). I Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the four groups. J , K Serum IL-10 and IL-22 levels in State fl/fl and Villin-Cre State fl/fl mice (with or without DSS treatment) were measured by enzyme-linked immunosorbent assay. * p < 0.05, ** p < 0.01, and **** p < 0.0001 compared with control groups.
    Figure Legend Snippet: A To determine the optimal LPS concentration, LPS was used to induce cellular inflammation in the human colonic epithelial cell line, NCM460. B After using lentivirus shSTAT3 to interfere with the expression of STAT3 in NCM460 cells, cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. C Immunofluorescence was performed to examine the expression of ZO-1 protein in NC and STAT3 low-expressing NCM460 cells with LPS treatment. D DSS was used to induce colitis in State fl/fl and Villin-Cre State fl/fl mice ( n = 8 per group). Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( E ), DAI score ( F ), colon length ( G ), and histopathological score ( H ). Body weight changes in mice are expressed as a percentage of the initial body weight ( E ). I Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the four groups. J , K Serum IL-10 and IL-22 levels in State fl/fl and Villin-Cre State fl/fl mice (with or without DSS treatment) were measured by enzyme-linked immunosorbent assay. * p < 0.05, ** p < 0.01, and **** p < 0.0001 compared with control groups.

    Techniques Used: Concentration Assay, Expressing, Cell Culture, Western Blot, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Control

    A Adeno-associated virus 8 (AAV8) expressing STAT3 (AAV– Stat3 ) was injected into mice to overexpress STAT3 and subsequently induce colitis in Usp25 −/− mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin (n = 8 per group). Induction of colitis in Usp25 −/− mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, STAT3, p-STAT3, ZO-1 and occludin in colons from the mice in two groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by enzyme-linked immunosorbent assay. ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with control groups.
    Figure Legend Snippet: A Adeno-associated virus 8 (AAV8) expressing STAT3 (AAV– Stat3 ) was injected into mice to overexpress STAT3 and subsequently induce colitis in Usp25 −/− mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin (n = 8 per group). Induction of colitis in Usp25 −/− mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, STAT3, p-STAT3, ZO-1 and occludin in colons from the mice in two groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by enzyme-linked immunosorbent assay. ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with control groups.

    Techniques Used: Virus, Expressing, Injection, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Control



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    A Adeno-associated virus 8 (AAV8) expressing Usp25 (AAV–Usp25) was injected into mice to overexpress USP25 and subsequently induce colitis in mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the mice in four groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by <t>enzyme-linked</t> <t>immunosorbent</t> <t>assay.</t> I After using the plasmid to overexpress USP25 in NCM460 cells, the cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. J Immunofluorescence was performed to examine the expression of occludin protein in NC and USP25 overexpressing NCM460 cells with LPS treatment. * p < 0.05, ** p < 0.01 compared with control groups.
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    A Adeno-associated virus 8 (AAV8) expressing Usp25 (AAV–Usp25) was injected into mice to overexpress USP25 and subsequently induce colitis in mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the mice in four groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by enzyme-linked immunosorbent assay. I After using the plasmid to overexpress USP25 in NCM460 cells, the cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. J Immunofluorescence was performed to examine the expression of occludin protein in NC and USP25 overexpressing NCM460 cells with LPS treatment. * p < 0.05, ** p < 0.01 compared with control groups.

    Journal: Cell Death & Disease

    Article Title: Ubiquitin-specific protease 25 ameliorates ulcerative colitis by regulating the degradation of phosphor-STAT3

    doi: 10.1038/s41419-024-07315-z

    Figure Lengend Snippet: A Adeno-associated virus 8 (AAV8) expressing Usp25 (AAV–Usp25) was injected into mice to overexpress USP25 and subsequently induce colitis in mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the mice in four groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by enzyme-linked immunosorbent assay. I After using the plasmid to overexpress USP25 in NCM460 cells, the cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. J Immunofluorescence was performed to examine the expression of occludin protein in NC and USP25 overexpressing NCM460 cells with LPS treatment. * p < 0.05, ** p < 0.01 compared with control groups.

    Article Snippet: Serum IL-10 and IL-22 levels were determined using mouse IL-10 and IL-22 enzyme-linked immunosorbent assay (ELISA) kits (Quantikine; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

    Techniques: Virus, Expressing, Injection, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Cell Culture, Immunofluorescence, Control

    A To determine the optimal LPS concentration, LPS was used to induce cellular inflammation in the human colonic epithelial cell line, NCM460. B After using lentivirus shSTAT3 to interfere with the expression of STAT3 in NCM460 cells, cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. C Immunofluorescence was performed to examine the expression of ZO-1 protein in NC and STAT3 low-expressing NCM460 cells with LPS treatment. D DSS was used to induce colitis in State fl/fl and Villin-Cre State fl/fl mice ( n = 8 per group). Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( E ), DAI score ( F ), colon length ( G ), and histopathological score ( H ). Body weight changes in mice are expressed as a percentage of the initial body weight ( E ). I Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the four groups. J , K Serum IL-10 and IL-22 levels in State fl/fl and Villin-Cre State fl/fl mice (with or without DSS treatment) were measured by enzyme-linked immunosorbent assay. * p < 0.05, ** p < 0.01, and **** p < 0.0001 compared with control groups.

    Journal: Cell Death & Disease

    Article Title: Ubiquitin-specific protease 25 ameliorates ulcerative colitis by regulating the degradation of phosphor-STAT3

    doi: 10.1038/s41419-024-07315-z

    Figure Lengend Snippet: A To determine the optimal LPS concentration, LPS was used to induce cellular inflammation in the human colonic epithelial cell line, NCM460. B After using lentivirus shSTAT3 to interfere with the expression of STAT3 in NCM460 cells, cells were co-cultured with LPS. Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin. C Immunofluorescence was performed to examine the expression of ZO-1 protein in NC and STAT3 low-expressing NCM460 cells with LPS treatment. D DSS was used to induce colitis in State fl/fl and Villin-Cre State fl/fl mice ( n = 8 per group). Colons were photographed and recorded and stained with hematoxylin and eosin ( n = 8 per group). Induction of colitis in mice was assessed by changes in mouse body weight ( E ), DAI score ( F ), colon length ( G ), and histopathological score ( H ). Body weight changes in mice are expressed as a percentage of the initial body weight ( E ). I Western blotting was performed to examine the protein expression levels of USP25, ZO-1 and occludin in colons from the four groups. J , K Serum IL-10 and IL-22 levels in State fl/fl and Villin-Cre State fl/fl mice (with or without DSS treatment) were measured by enzyme-linked immunosorbent assay. * p < 0.05, ** p < 0.01, and **** p < 0.0001 compared with control groups.

    Article Snippet: Serum IL-10 and IL-22 levels were determined using mouse IL-10 and IL-22 enzyme-linked immunosorbent assay (ELISA) kits (Quantikine; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

    Techniques: Concentration Assay, Expressing, Cell Culture, Western Blot, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Control

    A Adeno-associated virus 8 (AAV8) expressing STAT3 (AAV– Stat3 ) was injected into mice to overexpress STAT3 and subsequently induce colitis in Usp25 −/− mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin (n = 8 per group). Induction of colitis in Usp25 −/− mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, STAT3, p-STAT3, ZO-1 and occludin in colons from the mice in two groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by enzyme-linked immunosorbent assay. ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with control groups.

    Journal: Cell Death & Disease

    Article Title: Ubiquitin-specific protease 25 ameliorates ulcerative colitis by regulating the degradation of phosphor-STAT3

    doi: 10.1038/s41419-024-07315-z

    Figure Lengend Snippet: A Adeno-associated virus 8 (AAV8) expressing STAT3 (AAV– Stat3 ) was injected into mice to overexpress STAT3 and subsequently induce colitis in Usp25 −/− mice with DSS. Colons were photographed and recorded and stained with hematoxylin and eosin (n = 8 per group). Induction of colitis in Usp25 −/− mice was assessed by changes in mouse body weight ( B ), DAI score ( C ), colon length ( D ), and histopathological score ( E ). Body weight change in mice was expressed as a percentage of the initial body weight ( B ). F Western blotting was performed to examine the protein expression levels of USP25, STAT3, p-STAT3, ZO-1 and occludin in colons from the mice in two groups. G , H The levels of mouse serum inflammatory factors (IF) IL-10 and IL-22 were measured by enzyme-linked immunosorbent assay. ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with control groups.

    Article Snippet: Serum IL-10 and IL-22 levels were determined using mouse IL-10 and IL-22 enzyme-linked immunosorbent assay (ELISA) kits (Quantikine; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

    Techniques: Virus, Expressing, Injection, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Control